10/12/2023 0 Comments Gram negative rods in wound culture![]() Surgery of the integumentary system, p.161-259. Proper care of patient and antibiotic therapy may have good results, however the opposite also occurs, as overuse or misuse of antibiotics can result in development of antibiotic-resistant bacteria ( Hedlund 2007 Hedlund C.H. There are wounds which are not infected initially but have the potential to become so. Characteristics of bacteria that colonize and infect wounds, as pathogenicity and pattern of susceptibility to antimicrobials, can be affected by the wound care and use of topical and systemic antimicrobials. Factors such as type, quantity, and virulence of microorganisms and patient-linked aspects such as age, immunosuppression, and concomitant diseases influence the wound healing. Identificação da suscetibilidade antimicrobiana de bactérias isoladas de cães e gatos com feridas traumáticas contaminadas e infectadas. 2008 Arias M.V.B., Bataglia L.A., Aiello G., Carvalho T.T. Já fatores como nível sérico de lactato na admissão do paciente, pressão arterial média (PAM) e hematócrito não apresentaram correlação estatística com o óbito ou sobrevivência destes pacientes.Ĭultura de tecido hemocultura cães sepse bactérias bacteriemia glicemiaĬontaminated and infected wounds occur very frequently in veterinary medicine ( Arias et al. A hipoglicemia (≤60mg/dL) ou hiperglicemia severa (≥180mg/dL), também influenciaram negativamente a sobrevivência, pois 23,33% dos pacientes hipo/hiperglicêmicos vieram a óbito. Na hemocultura constatou-se bacteremia em sete pacientes, com predominância de bactérias Gram negativas, o que influenciou negativamente na sobrevivência dos pacientes, pois seis cães vieram a óbito. As bactérias gram positivas isoladas foram Streptococcus spp., Enterococcus spp. Foram isoladas bactérias de todas as feridas e a técnica de coleta de material para cultura por biópsia permitiu a obtenção de 41 agentes microbianos, sendo isoladas 53,66% bactérias Gram negativas e 46,34% Gram positivas, principalmente Pseudomonas aeruginosa, Klebsiella pneumoniae e Enterococcus spp. Os objetivos do presente trabalho foram verificar a viabilidade da técnica de coleta de material da ferida por biópsia para realização de cultura microbiana, determinar a frequência das bactérias nas culturas das feridas e hemoculturas e a susceptibilidade destes agentes aos antimicrobianos, bem como avaliar parâmetros clínicos que pudessem ser relacionados ao prognóstico em 30 cães com feridas e sinais de SIRS/sepse. ![]() Tissue culture hemoculture dogs sepsis bacteria bacteremia blood glucoseĪs feridas contaminadas e infectadas em cães ocorrem com grande frequência na medicina veterinária e podem causar síndrome da resposta inflamatória sistêmica, sepse e morte. ![]() Factors such as blood lactate level at admission and hematocrit levels, and mean arterial pressure were not significantly correlated with death or survival of the dogs. Hypoglycemia (≤60mg/dL) and severe hyperglycemia (≥180mg/dL) also negatively affected survival as 23.33% of the hypo/hyperglycemic dogs died. Based on blood culture analysis, we identified bacteremia in seven patients, predominantly of gram-negative bacteria, which negatively affected patient survival, as six dogs died. Bacteria were isolated from all wounds and 41 bacterial isolates could be identified based on culture of the materials collected by punch biopsy 53.66% of the isolates were gram-negative, mainly involving Pseudomonas aeruginosa, Klebsiella pneumoniae, and Enterococcus spp., and 46.34% were gram-positive bacteria such as Streptococcus spp., Enterococcus spp., and Staphylococcus spp. Thirty dogs with wounds and signs of SIRS/sepsis were included in this study. This study aimed to test the feasibility of collecting wound material by deep-tissue or punch biopsy for microbial culture, determine the frequency of bacteria in the wound(s) and blood cultures and the susceptibility of these microbes to antimicrobials, and evaluate clinical parameters that could be related to prognosis. Contaminated and infected wounds occur very frequently in veterinary medicine and can cause systemic inflammatory response syndrome, sepsis, and death.
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